Download PhoenIX™ Gigaprep Kit

PhoenIX™ Gigaprep Kit
1. Introduction
PhoenIX™ plasmid purification systems from Qbiogene rely on ion-exchange chromatography through a
unique, patented solid-phase column. Precisely balanced pH values and salt concentrations permit optimal
purification of highly clean plasmid DNA from the unique ion-exchange resin. Unlike resins found in other
ion-exchange kits, PhoenIX™ columns are distinct: First, the smaller pore size of the resin particles
increases the surface area available for binding. Second, the spacer molecule linking the positively charged
amino group to the resin particles is longer than in other resins, allowing a better degree of separation
between different biomolecules during the binding and washing process.
The PhoenIX™ Gigaprep Kit is driven by vacuum and offers lysate clearing via a filtration cartridge. The
PhoenIX™ Midiprep Kit and PhoenIX™ Maxiprep Kit rely on centrifugation to clear the bacterial lysate, and
on gravity to pull liquids through the column material.
Plasmid DNA purified with PhoenIX™ columns can be used immediately in a wide variety of downstream
applications including automated fluorescent sequencing, enzymatic amplification, labeling, transcription,
cloning and other enzymatic manipulations. Additionally, the levels of endotoxin that remain after purification are so low that successful transfection will occur with even sensitive cell lines.
2. Kit Components and User Supplied Materials
2.1 PhoenIX™ Gigaprep Kit Components
Description
RNase A (red cap)
Cell Resuspension Buffer (yellow cap label)
Lysis Buffer (blue cap label)
Neutralization Buffer (green cap label)
PhoenIX™ Giga Columns
Lysate Filtration Cartridges
Equilibration Solution (gray cap label)
Column Wash Buffer (orange cap label)
Elution Buffer (pink cap label)
User Manual
MSDS
Certificate of Analysis
Quantity
82.5 mg
650 ml
650 ml
650 ml
5 each
5 each
1025 ml
3 x 1010 ml
525 ml
1 each
1 each
1 each
Any Questions? Call Technical Support at (800) 424-6101
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