Download PhoenIX™ Maxiprep Kit

PhoenIX™ Maxiprep Kit
1. Introduction
PhoenIX™ plasmid purification systems from Qbiogene rely on ion-exchange chromatography through a
unique, patented solid-phase column. Precisely balanced pH values and salt concentrations permit optimal
purification of highly clean plasmid DNA from the unique ion-exchange resin. Unlike resins found in other
ion-exchange kits, PhoenIX™ columns are distinct: First, the smaller pore size of the resin particles
increases the surface area available for binding. Second, the spacer molecule linking the positively charged
amino group to the resin particles is longer than in other resins, allowing a better degree of separation
between different biomolecules during the binding and washing process.
The PhoenIX™ Midiprep Kit and PhoenIX™ Maxiprep Kit rely on centrifugation to clear the bacterial lysate,
and on gravity to pull liquids through the column material. The PhoenIX™ Gigaprep Kit is driven by vacuum
and offers lysate clearing via a filtration cartridge.
Plasmid DNA purified with PhoenIX™ columns can be used immediately in a wide variety of downstream
applications including automated fluorescent sequencing, enzymatic amplification, labeling, transcription,
cloning and other enzymatic manipulations. Additionally, the levels of endotoxin that remain after purification are so low that successful transfection will occur with even sensitive cell lines.
Two protocols are included in this instruction manual. The “high copy” protocol allows isolation of plasmid
DNA from bacterial cultures of 100 ml to 500 ml. The “low copy” protocol allows isolation of plasmid DNA
from bacterial cultures of 201 ml to 500 ml. In either protocol, plasmid DNA is ready to precipitate in just
over an hour.
2. Kit Components and User Supplied Materials
2.1 PhoenIX™ Maxiprep Kit Components
Description
RNase A (red cap)
Cell Resuspension Buffer (yellow cap label)
Lysis Buffer (blue cap label)
Neutralization Buffer (green cap label)
PhoenIX™ Maxi Columns
Disposable Maxi Column Rack
Equilibration Buffer (gray cap label)
Column Wash Buffer (orange cap label)
Elution Buffer (pink cap label)
User Manual
MSDS
Certificate of Analysis
Quantity
33 mg
260 ml
260 ml
260 ml
25 each
1 each
780 ml
775 ml
400 ml
1 each
1 each
1 each
Any Questions? Call Technical Support at (800) 424-6101
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