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Using Rotors Vertical-Tube and Near-Vertical Tube Rotors Rotor Preparation and Loading For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. Prerun Safety Checks Read all safety information in the rotor manual before using the rotor. 1 Make sure that the rotor, plugs, gaskets, and spacers are clean and show no signs of corrosion or cracking. • The high forces generated in these rotors can cause damaged components to fail. 2 Check the chemical compatibilities of all materials used. • (Refer to APPENDIX A.) 3 Verify that tubes and accessories being used are listed in the applicable rotor manual. Rotor Preparation and Loading 1 Be sure that plug threads are clean and lightly but evenly lubricated with Spinkote lubricant (306812). Threads Gasket 2 Set the rotor into the vise, which should be bolted or clamped to a rigid surface. 3 Dry the exterior of the plugged (OptiSeal) or sealed (Quick-Seal) tubes. • (Moisture between the tube and the rotor cavity may lead to tube collapse and increase the force required to extract the tube.) a. Slide the tubes into the tube cavities. • Tubes must be arranged symmetrically in the rotor (see Figure 1.5). TLR-IM-9AC 4-13 4