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oligoprop
use property name/property value pairs. You can specify one or more
properties in any order. Each PropertyName must be enclosed in single
quotation marks and is case insensitive. These property name/property
value pairs are as follows:
SeqProperties = oligoprop(SeqNT, ...'Salt', SaltValue, ...)
specifies a salt concentration in moles/liter for melting temperature
calculations. Default is 0.05 moles/liter.
SeqProperties = oligoprop(SeqNT, ...'Temp', TempValue, ...)
specifies the temperature in degrees Celsius for nearest-neighbor
calculations of free energy. Default is 25 degrees Celsius.
SeqProperties = oligoprop(SeqNT, ...'Primerconc',
PrimerconcValue, ...) specifies the concentration in moles/liter for
melting temperatures. Default is 50e-6 moles/liter.
SeqProperties = oligoprop(SeqNT, ...'HPBase', HPBaseValue,
...) specifies the minimum number of paired bases that form the neck
of the hairpin. Default is 4 base pairs.
SeqProperties = oligoprop(SeqNT, ...'HPLoop', HPLoopValue,
...) specifies the minimum number of bases that form the loop of a
hairpin. Default is 2 bases.
SeqProperties = oligoprop(SeqNT, ...'Dimerlength',
DimerlengthValue, ...) specifies the minimum number of aligned
bases between the sequence and its reverse. Default is 4 bases.
Examples
Calculating Properties for a DNA Sequence
1 Create a random sequence.
seq = randseq(25)
seq =
TAGCTTCATCGTTGACTTCTACTAA
2 Calculate sequence properties of the sequence.
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