Download Temperature Verification System Overview 1

Temperature Display The sample temperature display on the instrument will be different than the display on
Differences the digital thermometer during heating or cooling transitions. This is because the
digital thermometer measures block temperature while the thermal cycler measures
sample temperature. The instrument sample temperature display is a function of the
tube type and the reaction volume.
Using the Probe We recommend that you use the RTD probe and digital thermometer only for the
While Running temperature calibration verification test and the temperature uniformity test, which are
Programs described in these instructions on page 1-2. If you use the RTD probe and digital
thermometer while running programs other than those used in the two tests, be aware
that the accuracy of the RTD probe will decrease due to the effect of the heated
sample block cover.
The heated cover normally operates at 105 °C. The effect of the heated cover on the
RTD probe decreases as the temperature of the sample block approaches 99.9 °C.
Temperature Verification System Overview 1-5