Download Agilent 1200 Series Fluorescence Detector

Maintenance
10
Wavelength Calibration Procedure
When required
If application requires, or after replacement of flow cell or lamp.
Tools required
Laboratory balance
Parts required
Glycogen Calibration Sample, Syringe, needle, sample filter and PEEK fitting
from the Accessory Kit, see “Accessory Kit” on page 263
Steps
1 Preparation of the Glycogen Calibration Sample.
2 Preparation of the Flow Cell.
3 Wavelength Calibration.
4 Verification using “Check of Raman Band of Water” on page 156.
Preparation of the Glycogen Calibration Sample
1 To prepare 10 ml of the calibration solution you have to use 10 mg of the
Glycogen sample (a tolerance of ±20% is not critical).
2 Fill the prepared amount into a suitable bottle/vial.
3 Fill 10 ml of distilled water into the vial and shake.
4 Wait 5 minutes and shake again. After 10 minutes the solution is ready.
1200 Series FLD Service Manual
191