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Peak Fit Analysis Settings
page 361
Range Settings
•
Minimum - The pI value below which peaks will not be identified. This value will also be used as the
default lower pI range for the data displayed in the electropherogram and virtual blot. The default
value is 5.
•
Maximum: The pI value above which peaks will not be identified. This value will also be used as the
default upper pI range for the data displayed in the electropherogram and virtual blot. The default
value is 7.
Baseline Settings
•
Threshold - The variance, or roughness, in a baseline data segment below which a point will be
called part of the baseline. The default value is 1.0.
•
Window - How long baseline data segments are expected to be in pixels. Shorter segments will allow
the baseline to follow plateau sections of the signal. The default value is 15.
•
Stiffness - The amount the baseline is allowed to vary from a straight line. Settings between 0.1 and
1.0 make the baseline fit closer to a straight line. Settings from 1.0 to 10.0 will make the baseline fit follow the data more closely. The default value is 1.0.
Peak Find Settings
•
Threshold - The minimum signal to noise ratio required for a peak to be identified. A setting of 1.0 will
detect many peaks, a setting of 30.0 will detect fewer peaks. The default value is 20.0.
•
Width - The approximate peak width (at full width half max) in pixels used to detect peaks. The minimum value for this setting is 3.0. Larger widths help to eliminate the detection of shoulder peaks and
noise peaks. The default value is 7.0.
Peak Fit Analysis Settings Groups
Peak fit settings are saved as a group, and multiple settings groups can be created. Specific group settings
can then be applied to individual capillaries, sample names or attributes in the run data.
NOTES:
ProteinSimple recommends using the Compass default values for peak fit analysis settings. These settings
are included in the default Peak Fit group.
Analysis settings are run-file specific. However, settings can be imported or exported for use with other run
files. For more information see “Importing and Exporting Analysis Settings” on page 386.
Compass Software for Peggy User Guide