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page 6
Chapter 1: General Information
Waste Production and Disposal
It is the responsibility of the user to properly dispose of waste. The aqueous bulk waste stream is approximately pH 8. For reference, ProteinSimple disposes of waste under the non-RCRA Hazardous Waste Liquid
code 141 (buffer containing sodium chloride surfactants). A typical waste profile for one cycle (12 capillaries)
is as follows:
NOTE: Volumes should be adjusted accordingly to account for running of additional cycles.
.In
Waste Origin: Size-based Assays
Volume
Trough Reagents for 1 cycle
Matrix Removal Buffer
2.0 mL
Wash Buffer
2.0 mL
Running Buffer
1.6 mL
Water (for trough and manifold cleaning)
80.0 mL
Total waste per cycle
85.6 mL
Assay Plate Reagents*
Separation Matrix
0.240 mL
Stacking Matrix
0.240 mL
Biotinylated Ladder
0.005 mL
Antibody Diluent
0.200 mL
Primary Antibodies in Antibody Diluent
0.180 mL
Streptavidin-HRP
0.010 mL
Secondary HRP Conjugate
0.180 mL
Luminol/Peroxide Mix
0.180 mL
Prepared Sample – contains Sample Buffer,
DTT, Fluorescent Standards and Water
0.055 mL
*Assumes 1 row of sample and 1 row of antibody.
Peggy User Guide