Download Successful HPLC Operation

Table of Contents
1. Introduction
1.1 Purpose
1.2 Content
2. Troubleshooting Strategy and Processes
2.1 Strategy
2.2 Troubleshooting Process
3. Troubleshooting Isolation and Corrective Action
4
4
5
5
5
6
3.1 Visual Inspection
3.2 Pressure
3.2.1 High Pressure
3.2.2 Low Pressure
3.2.3 Fluctuating Pressure
3.3 Baseline Irregularities
3.3.1 Non-Cyclic Noise – Fluid Path Problems
3.3.2 Non-Cyclic Noise – Detector Electronics Problems
3.3.3 Cyclic Noise – Detector Related Problems and Others
3.4 Changes In Chromatography
3.4.1 Retention Time Changes from Injection to Injection
3.4.2 Continually Increasing or Decreasing Retention Times
3.4.3 Increasing or Decreasing to a New Constant Retention Time
3.4.4 Abnormal Peak Shape
3.5 Qualitative Results
3.6 Quantitative Results
6
6
7
8
9
10
10
12
13
14
14
16
17
18
27
29
4. Good Laboratory Practice for HPLC
31
4.1 Preparation of Solvents
4.1.1 Quality
4.1.2 Buffers
4.1.3 Filtration
4.1.4 Degassing
4.2 Solvent Use
4.2.1 Instrument
4.2.2 Mobile Phase Properties
4.3 Changing Solvents
31
31
31
31
32
32
32
33
33
4.3.1 Buffered Phase to Wash or Storage Phase
4.3.2 Normal to Reversed Phase and Vice Versa
4.3.3 General
2
4
33
33
34