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Image Analysis
Image preparation - Step-by-step
4) Select the Multiplicative option in the Assumed deterioration group.
5) Select the Source 1 option in the Source for shading image group to have
the correction image calculated based on the image being corrected.
6) Select the NxN average filter in the Preparation of shading image group.
7) Set the parameters for this filter in the NxN average filter group: e. g., set
the number of iterations to 3 and the size of the filter matrix at 50.
• Should you be working with true-color images, select the Polynomial
fit command.
8) Click the Execute button to apply shading correction to the image.
" The corrected image will be written into the destination image buffer.
• You have thus also defined parameters for further shading corrections. Simply select the Oper > Shading Correction command to conduct them.
The upper-left image shows
spherical polystyrene structures. Both the spherical
structures and image background get darker looking
from left to right. It is therefore not possible to set
thresholds only for particles.
After shading correction (upper right), image illumination
is even and thresholds can
thus be set.
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Separating particles automatically
1) Set the zoom level of the image in the viewport to 100%.
2) Select the Oper > Define Filter Separator... command.
3) Select the Step option in the Boundary shape group.
• The particle separator can also be used to track bright or dark lines.
Select the Dark or Bright option in this case.
4) Set both Smooth and Fine/Coarse slide controls to 1.