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Image Analysis
Classifying particles - Step-by-step
• Values in the results sheet can be shown in the color of their respective
class.
• You can have all shape factors greater than 1 set to 1.
Step-by-step
Defining a classification scheme
1) Select the Measure > Define Classification... command to define a classification scheme the way you need it.
2) Select the Show sample objects check box to be able to judge the size necessary for the classification.
" The current overlay will disappear from view and your image analysis
program will show a number of sample objects for visual reference
purposes.
3) Enter the name of the classification into the New Classification field.
4) Click the New button to create the new classification scheme.
5) Click the Unit... button to select the unit desired for the classification. The
unit you select will depend on the classification criterion. If you’re planning
on classifying according to area, for example, you could select a unit of
µm².
6) Click the Compute... button to define the number and value range of each
class. The value range is determined by the minimum and maximum particle parameters.
• If you have already detected an image, you can allow a suitable classification scheme to be automatically calculated: In the Compute
Classification dialog box, enter the criteria and the amount of classes
and click the Auto button.
7) Click the OK button to return to the Define Classification dialog box.
9) Click on OK to exit the dialog box.
Applying classification
1) Select the Analysis > Classify... command to divide detected particles up
into classes.
2) Select the particle parameter you wish to use to classify with in the Criteria
list in the Classify group: e. g., "Area". The parameter you use to classify
with is entirely up to you.
• Select No classification in the list of classification criteria if you do not
wish to have particles divided up into classes. The class parameters
will then refer to all the particles that are detected.
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8) If you wish to edit the class divisions interactively, go to the Define Classification dialog box:
• To alter the color of a class select the line number of that class and
select one of the 16 colors available in the Set color color palette.
• To give names to classes, leftclick in the Name field of the class Enter
the class name into this field.
• To interactively alter class divisions of a class, doubleclick on one of
the numeric values and enter a new one.