Download HPLC Troubleshooting Guide - Sigma

Filters
A precolumn filter is essential for protecting HPLC columns against particulate matter which can accumulate on the column frit, leading
to split peaks and high back pressure. Sources of particles include mobile phases (especially when buffers are mixed with organic solvents),
pump and injector seals, and samples. Use a 2.0 μm frit to protect columns containing 5 μm or larger particles, or a 0.5 μm frit for columns
with particles smaller than 5 μm.
Valco Precolumn Frit and
Screen Filters3
Supelco Filter
Direct-connect; protects analytical and
guard columns. Our precolumn filter can be
connected directly, hand-tight, into any HPLC
column or guard column listed in our current
catalog, or with any other column that has
Valco-compatible endfittings. PEEK cap and
body, 2 μm stainless steel frit. For a metal-free
system, order PEEK/Teflon replacement frits
(Cat. No. 57430-U).
Description
Supelco Precolumn Filter
Frits (pk. of 5)
0.5 μm pores
2 μm pore
PEEK/Teflon, 2 μm1
1
P000551
Cat. No.
Z22732-3
Z29087-4
Z22733-1
57430-U
Biocompatible, metal-free.
In-line installation. Efficient,
low dead volume filters protect
your columns from particles
without reducing column performance. The replaceable
P000550
1/8" frit has 0.5 μm pores to
protect 3 μm or 5 μm column packings, the replaceable screen has
2 μm pores. Choose the frit filter for higher filtration capacity (most
applications) or the screen filter for less dead volume (e.g., with
microbore columns). Use with 1/16" O.D. tubing; 1/16" fittings
included.
Description
Valco Precolumn Filters
Frit Filter
Screen Filter
Frits (pk. of 10)
0.5 μm pores
2.0 μm pores
Screens (pk. of 10)
3
Cat. No.
58420-U
58279-U
59037
59129
58284
Frits and screens should not be interchanged in these filters.
P000548
SSI High Pressure Precolumn Filter
In-line installation. The 316 stainless steel filter disc (0.5 μm pores)
is easily replaced without removing the column end fitting. Maximum operating pressure: 15,000 psi (1054 kg/cm²). For 1/16"
tubing.
Description
SSI High Pressure Precolumn Filter
10-32 Threads2
Waters Threads
Filter Elements and Seals (pk. of 10)
0.5 μm pores
2 μm pores
2
Cat. No.
59269
59271
59273
59272
Most HPLC fittings, except SSI and some Waters fittings, have 10-32 threads.
SSI High Pressure
Preinjector Filter
Place between the pump
and injector to provide final filtration for the mobile phase. Easily replaced
P000547
316 stainless steel filter
element (0.5 μm pores). Maximum operating pressure: 15,000 psi
(105MPa). For 1/16" O.D. tubing, 10-32 threads2.
Description
SSI High Pressure Preinjector Filter
Replacement Filter Elements and Seals (pk. of 2)
0.5 μm pores
2 μm pores
2
Cat. No.
59262-U
59264
59265
Most HPLC fittings, except SSI and some Waters fittings, have 10-32 threads.
18
Isolation
Technologies
Precolumn Filter
In-line installation.
P000549
High capacity inlet filter minimizes dead volume and band broadening, to prevent loss of
column efficiency while protecting your column. Frit porosity: 0.5
μm. Complete as shown.
Description
Isolation Technologies Precolumn Filter
with 3 mm frit (4.6 mm columns)
with 1.5 mm frit (2.1 mm columns)
Frits (pk. of 5)
3 mm
1.5 mm
Cat. No.
57675-U
57676-U
57677
57678
Upchurch Precolumn Filter
In-line installation. Stainless steel
body with inert polyetheretherketone (PEEK) endfittings and a
0.5 μm or 2 μm PEEK frit in one
endfitting.
Description
Upchurch Precolumn Filter
0.5 μm frit
2 μm frit
Frits (pk. of 10)
0.5 μm
2 μm
P000555
Cat. No.
55079
55078
55080-U
55081