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A second new method in the 750TOBS is to perform a constant wash at a preset flow rate. In the 750TOBS the fresh warm buffer from the reservoir is filled from below and excess buffer is evacuated from the overflow on the top. The flow rate can be programmed from 0.5 - 5.0 ml/min . Although it may take a little more time to completely wash any drugs away, the advantages are that the tissue is never in air, stays in warm buffer and stimulation, if used, does not need to be interrupted. The user is encouraged to try both methods and based on own judgment determine what is best for the experimental conditions. When the Constant wash mode is selected go to Setup->Chamber 1-4 to adjust the settings. 62 Tissue organ bath system Model 750TOBS