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E-Z 96® MagSi DNA Tissue DNA Kit for Buccal Swabs
The following protocol is designed for isolating DNA from buccal swabs with
magnetic beads.
Kit Contents
Product Number
M6229-00
M6229-01
M6229-02
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
500 :l Micro Plate
1
4
20
1.2ml Process Plate
1
4
20
1.
Place the swab into each well of 96 well plate(no provided).
2.
Prepare a Proteinase K/Buffer TL working solution by mixing 20 :l
Proteinase K with 250:l Buffer TL for each sample. For each 96 sets of
sample, prepare the Protease/TL stock working solution by mix 2.0mL
protease with 25mL Buffer TL. Pipet 250 :l protease/TL working solution
into each well. Seal the plate properly.
8-strip caps
10 x 8
35 x 8
170 x 8
MagSi DNA Particles
2.2 ml
9 ml
45ml
Buffer TL
20 ml
90 ml
2 x 180 ml
Buffer MSL
20 ml
90 ml
2 x 180 ml
Incubate at 56°C for 1 hours. Mix occasionally during the incubation by
rotating the plate gently. Make sure the samples are completely lysed.
Buffer MP
30 ml
100 ml
8 x 100 ml
2 ml
8 ml
32 ml
3.
Proteinase K
4.
Spin at 3,000-5,000 x g for 1 minutes at room temperature.
RNase A
550 :l
2.1 ml
10 ml
5.
Transfer 150 :l of the Sample into each well of Plate (provided).
Elution Buffer
100 ml
2 x 200 ml
8 x 250 ml
Add 150:l Buffer MSL, 20 :l MagSi DNA Paritcles and 220 :l absolute
ethanol to the lysate. Mix the sample by pipetting up and down 20-30 times
to mix well or shaking to mix well.
Note: Buffer MSL, MagSi DNA Particles and absolute ethanol can be
premixed. The mixture can be stored at room temperature for 3 hours.
1
1
1
6.
7.
Place the Plate on a magnetic separation device (MSD-01) to magnetize
the MagSi DNA particles for 3 min. The liquid should be cleared after all the
magnetic beads are pelleted on the corner of the each well adjecent to the
magnet.
8.
Aspirate and discard the cleared supernatant.
9.
Remove the Plate containing the MagSi DNA particles from the magnetic
separation device. Add 500:l Buffer MP diluted with ethanol into each
well.
Note: Buffer MP must be diluted with absolute ethanol (96-100%) before use
in this protocol.
Instruction Booklet
®
10. Resuspend MagSi DNA particles pellet by pipetting up and down 20 times or
shaking to mix well.
10
3