Download CASE Kit User Manual

Transcript
SuperArray CASE™ Kit User Manual
Version 2.2
10/14/2005
19
2. Large air bubbles in the 96-well plate can significantly and artificially inflate your
OD readings, especially when using the 1% SDS solution. To avoid the
appearance of air bubbles, remove a sufficient volume of every solution from the
washing buffer reservoir, avoid pipetting air bubbles, and examine the plate
before placing into your ELISA plate reader. Carefully break any air bubbles with
a pipet tip.
3. Improper antibody incubation and washing: Be sure to fill all wells with the
appropriate antibody and follow the recommendations for washing. Use a rocker
in every wash step.
H. No antibody solution in wells after overnight incubation:
Be sure to seal the open plate with sealing tape then with a piece of parafilm and
place the lid onto the plate. The plate may also be stored in a zip-lock or heat-sealed
bag. Be sure to store the plate on a level, stable surface.
If you have additional questions, please check our website (www.superarray.com)
for a more complete listing of Frequently Asked Questions (FAQs), or call our
Technical Support Representatives at 0800-2466651 or +49-40-853260-27,-37,-23.
Corning® and CellBIND® are registered trademarks of Corning Incorporated.
0800-2466651
Technical Support
+49-40-853260-23,-27,-37
[email protected] www.biomol.de www.SuperArray.com