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Perform a TruSeq v3 Run
Load Reagents for Read 2
After completion of Read 1 and any index reads, load paired-end reagents for Read 2
resynthesis and freshly prepared ICB for Read 2.
Load Paired-End Reagents
1
Record the weight of each reagent on the lab tracking form.
2
Make sure that the paired-end rack is not in use on the opposite flow cell for Read 2
resynthesis, Index 1 (i7) Read preparation, or Index 2 (i5) Read preparation.
3
Raise the sippers for the paired-end reagent rack using the following motion:
a Pull the handle towards you and raise the handle.
b Release the handle into the slot on the top end of the groove. Make sure that the
handle rests securely in the slot.
4
Slide the reagent rack out of the reagent compartment using the rack handle.
5
Remove the caps from each reagent tube.
6
Place each of the reagent tubes onto the rack in the associated numbered positions.
Table 9 Paired-End Reagent Positions
Position
Reagent
10
11
12
13
14
15
16
18
19
RMR
LMX2
BMX
AMX2
APM2
AT2
HP7 or HP11
HP3
HT2
Description
Resynthesis Mix
Linearization Mix 2
Blocking Mix
Amplification Mix 2
AMX2 Premix
100% Formamide
Read 2 Sequencing Primer
Denaturation Solution
Wash Buffer
NOTE
For dual-indexed paired-end runs, RMR is loaded with indexing reagents before starting
the run. For single-indexed or non-indexed runs, RMR is loaded with paired-end
reagents.
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Part # 15035786 Rev. D