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Analyzing and Processing Data Table 6-4 Chromatograms (Continued) Types of chromatograms Purpose BPC (Base Peak Chromatogram) Chromatographic plot that shows the intensity of the most intense ion within a scan versus time or scan number. TWC (Total Wavelength Chromatogram) A chromatographic display created by summing all of the absorbance values in the acquired wavelength range and then plotting the values against time. It consists of the summed absorbances of all ions in a scan plotted against time in a chromatographic pane. XWC (Extracted Wavelength Chromatogram) A subset of TWC, an XWC shows the absorbance for a single wavelength or the sum of the absorbance for a range of wavelengths. DAD (Diode Array Detector) A UV detector that monitors the absorption spectrum of eluting compounds at one or more wavelengths. Show TICs from a Spectrum Prerequisite: The Example project is selected. 1. In the Navigation Bar, under Explore, double-click Open Data File. The Select Sample dialog opens. 2. In the Data Files list, open a file containing spectra. 3. Click Explore > Show > Show TIC. The TIC opens in a new pane. Tip! TIC. Right-click inside a pane containing a spectrum and then click Show Show a Spectrum from a TIC 1. In a pane containing a TIC, select a range. 2. Click Explore > Show > Show Spectrum. The spectrum opens in a new pane. Tip! Double-click in the TIC pane at a particular time to show the spectrum. Generate XICs The user can generate XICs only from single period, single experiment chromatograms or spectra. To obtain an XIC from multi-period or multi-experiment data, split the data into separate panes by clicking the triangle that is under the x-axis. For more information about using the available icons, refer to Table 6-8. System User Guide D5033331 A AB SCIEX TripleTOF® 5600/5600+ Instruments 69 of 116