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Download JETQUICK Gel Extraction / PCR / General
Transcript
Table of Contents Kit Contents and Storage ....................................................................................... iv Kit Specifications ....................................................................................................vii PCR Purification .......................................................................... 1 System Overview...................................................................................................... 1 Experimental Overview .......................................................................................... 3 PCR Purification Procedure.................................................................................... 4 Troubleshooting........................................................................................................ 8 Gel Extraction .............................................................................. 9 System Overview...................................................................................................... 9 Experimental Overview ........................................................................................ 11 Gel Extraction Procedure ...................................................................................... 12 Troubleshooting...................................................................................................... 17 General DNA Clean-Up.............................................................. 18 System Overview.................................................................................................... 18 Experimental Overview ........................................................................................ 19 General DNA Clean-Up Procedure..................................................................... 20 Troubleshooting...................................................................................................... 24 Appendix .................................................................................... 25 Estimating DNA Yield and Quality .................................................................... 25 Accessory Products ................................................................................................ 27 Technical Support................................................................................................... 28 Purchaser Notification........................................................................................... 29 iii
