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Figure 3.6 Measuring the uniformity of illumination across the entire projector image
[117]. (a) Heat map showing the variation of intensity across the 1024x768 pixel
projector image. Black dotted line represents the field of view of the camera. The red
dotted circle represents the central region (~1mm diameter) where the worms are located
during active tracking of the animals. This demonstrates that the uniformity of the
projected illumination intensity is within 6% of the maximum. Knowing the calibration
factors, one can also correct/compensate during illumination if desired. (b) Variation of
intensity across the X axis at Y=384. Dotted red lines represent central region. (c)
Variation of intensity across the Y axis at X=512. Dotted red lines represent central
region.
3.3.3 Spatial Resolution and Accuracy
There are a number of factors involved in determination of system resolution and
accuracy. We will address each individually below.
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