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04 Applikon Biotechnology | Cultivation Systems from Discovery to Production Micro-Flask by Duetz, cultivation in microtiter plates The Micro-Flask system facilitates reproducible and reliable culturing on microtiter plates. The system consists of sandwich covers, cover clamps and cryo-replicator. The Micro-Flask enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handling. Cryo-Replicatorforsimpleinoculationof96-wellmicrotiterplates Features •Conversionof24and96microtiterplates(bothdeep-andlow-wellplates) into individual micro-reactors •Lowanduniformevaporationratesforeverywell O2 •Sterilebarrierforindividualwellspreventscrosscontamination •Oxygentransferratessimilartoshakeflasksinstandardorbitalshakers •Simultaneousandreproduciblesamplingof96frozenglycerolstocks H 2O + CO2 Applications •Highthroughputscreeninganddistributionofmutantandconstruct C U LTU RE libraries e.g. in E. coli or yeast •Metabolicfluxstudiesandhigh-throughputscreeningforhighactivity prokaryotic or eukaryotic mutants •Comparativestudies,e.g.clinicalisolates •Growthmediumoptimizationforcelllinesorproductionstrains Cross-section of Micro-Flask sandwich cover