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3.4 Using the “Tag” Function 3.4.1 After performing the counting function, the “Tag” button can be used to verify the counting results. Press Tag button Green circle – live cell Red circle – dead cell Note: This “Tag” function is one of the distinct tools of the Luna™ automated cell counter because it helps users review and determine the accuracy of the cell counting on-site without depending on a computer. 3.4.2 When touching the “Tag” button, the image on the screen will show objects (mostly cells) surrounded by green or red circles. The green circles indicate live cells and the red circles indicate dead cells. 3.4.3 After reviewing the accuracy of the image analysis, “Tag” button can be pressed again to remove the green and red circles. 21