Download A Microfluidic Device for Single Cell Isolation

Contents
Abstract ....................................................................................................................................................................................... 2
List of Figures ............................................................................................................................................................................ 6
List of Tables.............................................................................................................................................................................. 7
Authorship.................................................................................................................................................................................. 8
Acknowledgements ................................................................................................................................................................ 9
Chapter 1: Introduction ...................................................................................................................................................... 10
Chapter 2: Literature Review ........................................................................................................................................... 13
2.1 History of Cell Culture ............................................................................................................................................. 13
2.2 Growing field ............................................................................................................................................................... 14
2.3 Applications ................................................................................................................................................................. 15
2.4 Current single cell analysis devices ................................................................................................................... 16
2.4.1 Gold standard ..................................................................................................................................................... 16
2.4.2 Single Cell Isolation Methods ....................................................................................................................... 16
2.4.3 Limitations of Current Single Cell Analysis Techniques ................................................................... 20
2.5 Microfluidics ................................................................................................................................................................ 20
2.5.1 Why use microfluidics .................................................................................................................................... 20
Chapter 3: Project Strategy................................................................................................................................................ 23
3.1 Initial Client Statement ........................................................................................................................................... 23
3.2 Objectives ..................................................................................................................................................................... 23
3.3 Revised Client Statement: ...................................................................................................................................... 27
3.4 Project Approach:...................................................................................................................................................... 28
Chapter 4: Alternative Designs ........................................................................................................................................ 29
4.1 Needs Analysis............................................................................................................................................................ 29
4.2 Functions (Specifications) ..................................................................................................................................... 29
4.3 Conceptual Designs .................................................................................................................................................. 30
4.3.1 Alternative Design 1 ........................................................................................................................................ 30
4.3.2 Alternative Design 2 ........................................................................................................................................ 31
4.3.3 Alternative Design 3 ........................................................................................................................................ 33
4.3.4 Alternative Design 4 ........................................................................................................................................ 34
4.3.5 Alternative Design 5 ........................................................................................................................................ 35
4.4 Feasibility Study/ Experiments ........................................................................................................................... 36
4.5 Modeling ....................................................................................................................................................................... 36
4.5.1 Design Calculations .......................................................................................................................................... 36
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