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27. Leave the plate in the magnetic separator for the following wash steps:
a. Add 100 μL Assay Buffer to each well.
b. Use a multi-channel pipette to carefully aspirate the supernatant from
each well or use the manual inversion wash method. Take care not to
disturb the microspheres.
c. Repeat steps a and b above.
28. Remove the plate from the magnetic separator and resuspend the
microspheres in 100 μL of Assay Buffer by gently pipetting up and down
several times with a multi-channel pipettor.
29. Analyze 50–75 μL on the Luminex analyzer according to the system manual.
Capture Sandwich Immunoassay References:
• Bjerre M, Hansen TK, Flyvbjerg A, Tønnesen E. “Simultaneous detection of
porcine cytokines by multiplex analysis: development of magnetic bioplex
assay.” Vet Immunol Immunopathol. 2009;130:53-58.
Chapter 4.1.1 | Capture Sandwich Immunoassay
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