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Figure 5. Schematic Procedure
Assemble the Optimiser™ plate and the Optimiser™ pad in the Optimiser™ plate holder.
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Dispense 5 µL of capture antibody to the required number of wells in the Optimiser™ plate.
Incubate 10 minutes at room temperature (RT).
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Dispense 5 µL of OptiWash™ to the wells. Wait 10 minutes to proceed to the next step.
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Dispense 5 µL of OptiBlock™ to the wells. Incubate 10 minutes at RT.
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Dispense 5 µL of standard, control, sample, and blank to the wells. Incubate 20 minutes at RT.
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Dispense 5 µL of OptiWash™ to the wells. Wait 10 minutes to proceed to the next step.
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Dispense 5 µL of detection antibody to the wells. Incubate 10 minutes at RT.
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Dispense 5 µL of OptiWash™ to the wells. Wait 10 minutes to proceed to the next step.
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Dispense 5 µL of SAv-HRP to the wells. Incubate 10 minutes at RT.
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Dispense 30 µL of OptiWash™ to the wells. Wait 10 minutes to proceed to the next step.
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Again, dispense 30 µL OptiWash™ to the wells. Wait 10 minutes to proceed to the next step.
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Dispense 10 µL OptiGlow™ working solution to the wells. Incubate 15 minutes at RT.
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Determine the fluorescence of the wells using a microplate reader.
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