Download Cold Fusion User Manual

Cold Fusion™ Cloning Kit
Cat. # MC010A-1, MC 100A-1, MC 101A-1
Contents
I. Introduction .............................................................................2 A. Key Features .......................................................................2 B. Applications .........................................................................2 C. List of Components .............................................................3 D. Storage................................................................................4 E. Other Reagents Needed .....................................................4 F. Technical Information ..........................................................4 II. Protocol ...................................................................................5 A. Overview ................................................................................5 B. Preparation of Linearized Vector ...........................................5 C. Primer Design ........................................................................6 D. PCR Requirements ................................................................6 E. Cold Fusion Reaction ............................................................6 F. Transformation ......................................................................7 III. Examples .................................................................................8 A. Primer Design for Positive Control Reaction.........................8 B. Cloning a single DNA Fragment............................................8 C. Joining Multiple DNA Fragments ..........................................8 IV. Troubleshooting ......................................................................9 V. Technical Support .................................................................11 VI. Licensing and Warranty ........................................................11 888-266-5066 (Toll Free)
650-968-2200 (outside US)
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