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R E L A T I V E A R E A At the top of the page are parameters for specifying the relative area regions. In the middle is a pane containing a table of the relative area ranges and relative abundance percentages. Finally, the bottom pane displays the processed target spectrum with each area highlighted in color. Specifying ranges There are two ways to specify relative area ranges. 1. Click the + button in the upper left corner of the Mass Ranges to Analyze table. This adds a new row to the table. Type the m/z range start and end values. Continue until all ranges have been defined, and then click the Apply button. 2. Alternatively, you may right-click and drag in the spectrum window. Right-click at the beginning of the range, and then while holding the mouse button down, drag to the end of the range. Repeat this for each new range. The ranges will automatically be added to the table. If you wish, you may fine-tune the range setting by editing the table afterward. Click the Apply button to calculate the percentages. Relative Area results The relative areas and their percentages are displayed in the table in the middle pane. For each range, Polymerix computes the total integrated intensity, the percentage that intensity reflects of all of the defined ranges and of the spectrum as a whole (which may include area outside of defined ranges). 89