Download Biosafety Manual Draft Revision

Biosafety Manual
IBC-approved version (May 18, 2010)
•
•
Caution placards and other information must be posted at laboratory entrances,
including a biohazard label for BL2 work areas. See additional details in the next
paragraph.
Consult the Medical and Biohazardous Waste Generator’s Guide (PUB-3095) for details
on labels and colors for sharps containers, waste containers, and waste bags in
designated red-bag or clear-bag areas. A determination must be made in the Biosafety
Work Authorization as to whether the work will generate either regulated medical waste
(i.e., red-bag waste that is regulated by the California Department of Health Services) or
nonregulated biohazardous waste not (i.e., clear-bag waste).
The following criteria must be implemented when posting an entrance to BL1 or BL2 laboratory
area:
• A Caution placard must be posted at the entrance to a Technical Area as specified in the
LBNL CHSP.
• Area PPE requirements must be included on the Caution Placard as specified Chapter
19 of PUB-3000 (PPE).
• A biohazard label must be posted (typically on a placard) at the entrance to each BL2
work area to advise entering personnel of potential biological hazards.
• When infectious agents (i.e., human pathogens) are present or there are organisms that
require special provisions for entry (e.g., vaccination), additional biological hazard
warning signage is required at the entrance to the laboratory. This signage must
incorporate the universal biohazard symbol and include: the laboratory’s biosafety level;
the identity of the agent(s) or the words “Infectious Agent(s)”; the name and telephone
number of the supervisor, work lead, prinicipal investigator (PI), or other responsible
personnel; and any special requirements or procedures for entering and exiting the
laboratory. The CHSP Caution Placard will be used to accomplish these additional
signage requirements. Any requirements for posting identities of agents or posting
special entry and exit procedures will be specified in the BUA.
• Other LBNL requirements for signage (e.g., radiological) may also apply to the entrance
to be posted.
5.6
Facilities, Laboratory Equipment, and Related Practices
This section describes in a topical manner biosafety engineering and work practice controls
related to standard facility design and laboratory equipment. Properly designed and used
facilities, facility equipment, laboratory equipment, and lab tools provide protection for laboratory
workers, persons outside the laboratory, the public, and the environment.
See Sections 4.1, 4.2, and 4.3 of this manual for a general discussion of the principles of
standard laboratory practices, equipment, and facilities. See Appendix C of this manual for a
summary of standard laboratory practices, equipment, and facilities categorized as BL1 and
BL2. See PUB-3000, Section 26.7.6, for a discussion of the facility design process at LBNL
related to biosafety.
5.6.1
Cleanable Surfaces and Furnishings
In general, laboratory facilities and furnishings should be designed and maintained so that they
are durable, will not trap contamination, and can be easily cleaned. The following BL1 and BL2
laboratory criteria specified by BMBL and apply to this objective:
• The laboratory should be designed so that it can be easily cleaned or decontaminated.
Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version.
50