Download PROTOCOLS RNA Handling

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Contents
1
1.1
1.2
2
2.1
2.1.1
2.1.1.1
2.1.1.2
2.1.1.3
2.1.2
2.1.2.1
3
3.1
3.2
3.3
3.4
4
4.1
4.1.1
4.1.2
4.2
5
6
6.1
6.1.1
6.1.2
6.1.3
6.2
6.2.1
6.2.2
6.3
6.3.1
6.3.2
6.4
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7.1
7.1.1
7.1.2
7.2
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10.1
10.2
10.3
RNA handling – 0406
Introduction
Some introducing remarks on RNA
The DOs and DON’Ts on handling RNA
First steps of sample preparation
Preparation of slides
Samples on PALM MembraneSlides
Treatment to remove RNases
UV treatment
Poly-L-Lysine treatment
Samples on glass slides
Treatment to remove RNases
Mounting samples onto slides
Frozen sections
Paraffin embedded (FFPE) sections
Cytospins
Blood and tissue smear
Staining procedures
Frozen sections
Cresyl Violet
Hematoxylin/Eosin (HE)
Paraffin embedded (FFPE) sections
Storage
Laser Microdissection and Pressure Catapulting (LMPC)
Procedures
Tips for improvement of the morphological information
PALM Diffusor CM (#1210-0320)
PALM AdhesiveCaps (#1440-0240; #1440-0250)
PALM LiquidCover Glass N (#1440-0600)
Collection devices
PALM AdhesiveCaps (#1440-0240; #1440-0250, #1440-0245;
#1440-0255)
Other microfuge tubes
Collection procedures
“Dry” collection (PALM AdhesiveCaps)
“Liquid” collection (other microfuge tubes)
Capture check - looking into the cap to see the catapulted samples
Downstream Applications
Preparation of RNA
RNA from frozen sections
RNA from FFPE sections
Quality control of RNA
General remarks on RNA (distribution, content, RNase
activity)
PALM LabTips for working with RNA
Other protocols
DNA
Chromosomes
Live Cells
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