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Download CHP2 - Bulletin - Sigma
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1448 Fold Reduction in DNA Leakage from Staph-Seq 1600 Fold Reduction in Staph A DNA 1400 1200 1000 800 600 400 200 0 Staph A Staph-Seq Fig. 2 Staph A DNA contamination is reduced using ChIP Next Gen Seq Sepharose. Chips were performed using ChIP Next Gen Seq Sepharose versus untreated Staph A control cells and then analyzed using primers targeting Staph A rDNA. A 1448 fold reduction in Staph A DNA contamination (a 10.5 Ct difference) was observed using Workflow Cross-Link Cells in Suspension Prepare Nuclear Lysate Sonicate Chromatin Reverse XL, Determine DNA Concentration and Size Range Block Staph-Seq Preclear Chromatin IP Stringent Washes Elute IP Complexes Cross-Link Reversal Column DNA Purification Elute DNA qPCR WGA Library ChIP-chip ChIP-Seq 4
