Download E-PAGE

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Transcript 
Contents
Product Contents........................................................................................................................................ iv
Introduction .................................................................................................................................1
E-PAGE™ Protein Electrophoresis System ................................................................................................1
E-PAGE™ System Components ..................................................................................................................4
Methods .......................................................................................................................................9
Electrophoresis of E-PAGE™ Gels.........................................................................................9
Guidelines for Sample Preparation............................................................................................................9
Guidelines for Loading E-PAGE™ Gels by Automated Liquid Handling ..........................................14
Loading E-PAGE™ Gels .............................................................................................................................16
Using E-Holder™ Platform ........................................................................................................................20
Running E-PAGE™ Gels.............................................................................................................................21
Opening the E-PAGE™ Cassette ...............................................................................................................25
Blotting E-PAGE™ Gels.........................................................................................................26
Blotting E-PAGE™ Gels with the iBlot® Device ......................................................................................26
Semi-Dry Blotting of E-PAGE™ Gels........................................................................................................32
Semi-Wet Blotting of E-PAGE™ Gels .......................................................................................................36
Visualizing and Staining of E-PAGE™ Gels ........................................................................39
Visualizing Lumio Fusion Proteins .......................................................................................................39
SYPRO® Ruby Staining Protocol ..............................................................................................................40
Coomassie® Staining ..................................................................................................................................41
SimplyBlue™ SafeStain ...............................................................................................................................43
Silver Staining Methods.............................................................................................................................45
InVision™ His-tag Protein Gel Stain.........................................................................................................48
Drying of E-PAGE™ Gels ......................................................................................................49
Gel Drying ...................................................................................................................................................49
Analysis and Troubleshooting ............................................................................................51
Molecular Weight Calibration ..................................................................................................................51
Expected Results.........................................................................................................................................53
Using E-Editor™ 2.02 Software..................................................................................................................60
Troubleshooting..........................................................................................................................................61
Appendix....................................................................................................................................63
Additional Staining Protocols...................................................................................................................63
Product Specifications................................................................................................................................67
Accessory Products ....................................................................................................................................70
Technical Support.......................................................................................................................................72
Purchaser Notification ............................................................................. Error! Bookmark not defined.
iii
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