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Download NativePure pcDNA Gateway Vector Kit
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Additional Products Some of the reagents supplied in the NativePure™ pcDNA™ Gateway® Vector Kit, as well as other products suitable for use are available separately from Invitrogen. For more information, go to www.invitrogen.com or contact Technical Service (see page 36). Accessory Products Product ™ Amount Catalog no. ® 20 reactions K2400-20 ® pCR8/GW/TOPO TA Cloning Kit 20 reactions K2500-20 Gateway® LR Clonase™ II Enzyme Mix 20 reactions 100 reactions 11791-020 11791-100 One Shot® TOP10 Chemically Competent Cells 10 reactions C4040-10 One Shot® TOP10 Electrocompetent Cells 10 reactions C4040-50 One Shot® ccdB Survival T1R Chemically Competent Cells 10 reactions C7510-03 LB Media 500 ml 10855-021 Ampicillin 200 mg 11593-027 100 reactions K2100-01 0.75 ml 11668-027 20 ml 10131-035 Quant-iT Protein Assay Kit 1000 assays Q33210 Streptavidin-AP Conjugate 125 µl SA100-04 Streptavidin-HRP Conjugate pENTR /D-TOPO Cloning Kit PureLink™ HQ Plasmid Miniprep Kit ™ Lipofectamine 2000 ® Geneticin Selective Antibiotic, liquid ™ 2.5 mg 43-4323 ® 20 reactions WB7105 ® WesternBreeze Chemiluminescent Kit, Anti-Rabbit 20 reactions WB7104 NuPAGE® MOPS SDS Running Buffer (20X) WesternBreeze Chromogenic Kit, Anti-Rabbit 500 ml NP0001 ® 500 ml NP0002 ® NuPAGE LDS Sample Buffer (4X) 10 ml NP0007 NuPAGE® Sample Reducing Agent (10X) 250 µl NP0004 NuPAGE® Transfer Buffer (20X) 1L NP0006-1 HiMark Pre-Stained Protein Standard 250 µl LC5699 Tris-Glycine SDS Running Buffer (10X) 500 ml LC2675 Tris-Glycine SDS Sample Buffer (2X) 20 ml LC2676 Nitrocellulose (0.45 µm) Membrane/Filter Paper Sandwiches 20 sandwiches LC2001 NuPAGE MES SDS Running Buffer (20X) ™ ™ Invitrolon PVDF (0.45 µm) Membrane/Filter Paper Sandwiches 20 sandwiches LC2005 Phosphate-Buffered Saline (PBS), 1X 500 ml 10010-023 Anti-V5 Antibody 50 µl R960-25 Anti-V5-AP Antibody 125 µl R961-25 Anti-V5-HRP Antibody 50 µl R962-25 Continued on next page vii
