Download Lentivector Expression Systems:

Lentivector Expression Systems:
Guide to Packaging and Transduction of Target Cells
Cat. #s LV100A-1 – LV601B-1,
& LV900A-1
Contents
I.
Introduction and Background
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B.
C.
D.
E.
F.
G.
H.
Purpose of this Manual
Lentiviral Expression Systems
SBI’s Expression Lentivectors
Packaging of Expression Constructs into Pseudoviral Particles
Delivery of Packaged Lentivector Constructs into Target Cells
Safety Guidelines
List of Components
Additional Required Materials
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II. Protocol
A. Procedure Outline and General Comments
B. Pseudovirus Production
C. Pseudoviral Titer Estimation
D. Transduction of the Packaged Lentivector Expression Construct
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III. Troubleshooting
A. Low Viral Titer (<105 ifu/ml)
B. Inefficient Transduction of Packaged Constructs
IV. References
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V. Appendix
A.
B.
C.
D.
E.
F.
G.
Examples of SBI’s Expression Lentivectors
Functional Maps of Packaging Plasmids for FIV-based System
Functional Maps of Packaging Plasmids for HIV-based System
Percentage of Transduced Cells with Increasing MOI
Properties of copGFP Fluorescent Protein
Related Products
Technical Support
VI. Licensing and Warranty Statement
888-266-5066 (Toll Free)
650-968-2200 (outside US)
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