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Lentivector Expression Systems:
Guide to Packaging and Transduction of Target Cells
Cat. #s LV100A-1 – LV601B-1,
& LV900A-1
Mouse Cell Lines
RAW 264.7
(mouse leukaemic monocyte macrophage)
100%
100%
80%
80%
P19
(mouse embryo teratocarcinoma)
% infected cells
% infected cells
FIV-based pSIF1-copGFP
60%
40%
FIV-based pSIF1-copGFP
20%
HIV-based pSIH1-copGFP
60%
40%
20%
HIV-based pSIH1-copGFP
0%
0%
0
1
2
3
4
5
6
7
8
9
10
0
1
2
3
NB41
(mouse neuroblastoma)
100%
5
6
7
8
9
10 11 12 13 14 15
NIH3T3
(mouse embryonic fibroblast)
100%
80%
% infected cells
80%
% infected cells
4
Viral Titer (arbitrary units)
Viral Titer (arbitrary units)
60%
40%
FIV-based pSIF1-copGFP
20%
60%
40%
FIV-based pSIF1-copGFP
20%
HIV-based pSIH1-copGFP
HIV-based pSIH1-copGFP
0%
0%
0
1
2
3
4
5
6
7
8
0
9 10 11 12 13 14 15 16
1
2
P388
(mouse lymphocytic leukemia)
100%
3
4
5
6
7
8
9
10
50
55
Viral Titer (arbitrary units)
Viral Titer (arbitrary units)
mouse Lin- ckit+ bone marrow
stem cells
100%
FIV-based pSIF1-copGFP
HIV-based pSIH1-copGFP
% infected cells
% infected cells
80%
FIV-based pSIF1-copGFP
80%
60%
40%
20%
0%
HIV-based pSIH1-copGFP
60%
40%
20%
0%
0
1
2
3
4
5
6
7
8
9
10
Viral Titer (arbitrary units)
888-266-5066 (Toll Free)
650-968-2200 (outside US)
0
5
10
15
20
25
30
35
40
45
Viral Titer (arbitrary units)
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