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ml scale mark, and the other one is cut very close to the tip. The tip portions of the
syringes are glued together using a hot melt glue gun. One end of the reservoir is
connected to the solution line, and the other end is connected to a solution switch.
This switch is used to hold the air pressure, and when it's turned on, the pressure
is released.
3. Shield everything
Since the solution lines behave like an antenna network, it is import to shield the
exposed lines as much as possible. You may make a Faraday cage around the
perfusion system using aluminum foil or plate, and make sure that the Faraday
cage is grounded. If the cage is not grounded, it might introduce even more noise
into the system.
In addition to shielding the solution lines, you may want to shield the temperature
control system as well. Although the circulating water in the temperature control
system is not electrically connected to the recording chamber, it may still carry
detectable noise into the recording system. Use aluminum foil to wrap all the
tubings, and again, make sure that the foil is properly grounded.
There is still something more to be shielded. Tubings for hydraulic
micromanipulator (if used), the headstage for recording amperometric current,
and the carbon electrode (except the portion that is inserted into the pipette
holder) also need to be shielded. It is to reduce the noise in amperometric current.
4. Ground yourself