Download as a PDF

The reader may notice that in figure 22 the percent of matched spots within the
gels are fairly low values. These values are still acceptable because this number is the
percent of matched spots out of the total number of spots on the sample gel, not the
universal standard gel. In the future it may be beneficial to calculate the percent of
matched spots based on the spots present in the universal standard gel. By calculating the
variable based on the number of spots in the universal standard gel, the percent of
matched spots should be greatly increased. Also, this proposed method of calculation
will allow future investigators to establish a value for the percent of matched spots that
all replicate gels must agree with. For example, any future gels must match 95% of the
spots present on the universal standard gel to be within acceptable statistical limits of
matching.
These values of %CV and the percent of matched spots in a gel are different than
the values displayed in figure 13, because this experiment uses the universal gel standard
as the reference gel in the analysis. In the first analysis, the reference gel was assigned
by the software, which automatically makes the gel that contains the most spots the
reference gel. This comparison to the universal standard gel is more accurate because it
compares three gels instead of two. Both of these variables still agree with the trends
observed in the first intra-set comparisons. The percent of matched spots are highest at
5.5 and 6 hours, indicating that the locations of the spots on the gels are similar to each
other. The %CV value is again lowest when comparing the gels that had similar growth
times to mid-log phase to the universal standard gel.
48