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List of Figures
Figure 1: Isoelectric Focusing Schematic – Page 5
Figure 2: Structure of Sodium Dodecyl Sulfate – Page 6
Figure 3: Components of a 2DE System – Page 7
Figure 4: Growth Phases of a Bacterial Lifecycle – Page 9
Figure 5: Four Corners Method of Plate Streaking – Page 11
Figure 6: Sample Placement in an IEF Tray – Page 18
Figure 7: Protean IEF Cell – Page 19
Figure 8: Impurities Commonly Found on Gels – Page 23
Figure 9: Matching and Warping of the Gels – Page 24
Figure 10: Growth Curves of P. putidaKT2440 – Page 27
Figure 11: Average Growth Curve for P. putidaKT2440 – Page 27
Figure 12: Representative Gels from Each Time Point of Log Phase – Page 30
Figure 13: Results of the Intra-set Gel Comparison – Page 32
Figure 14: Representations of the Universal Standard Gel – Page 34
Figure 15: Histogram and Montage Window for Spot 142 – Page 36
Figure 16: Histogram and Montage Window for Spot 75 – Page 37
Figure 17: Histogram and Montage Window for Spot 87 – Page 38
Figure 18: Histogram and Montage Window for Spot 40 – Page 39
Figure 19: Expression Table for a Twofold Change – Page 42
Figure 20: Percent of Spots that Exhibit Fold Changes – Page 44
Figure 21: Coefficients of Variation Compared to the Universal Standard Gel – Page 45
Figure 22: Percent of Matching Spots to the Universal Standard Gel – Page 45
Figure 23: Comparison of the Intra-set and Universal Standard gel Analyses – Page 46
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