Download Chapter 1 - Worcester Polytechnic Institute

4.2 Conceptual Designs ........................................................................................................................... 34
4.2.1. Mechanical conditioning system .............................................................................................. 34
4.2.2. Tissue chamber ......................................................................................................................... 37
4.2.3. Design assembly ........................................................................................................................ 39
4.3 Comparison of Design Components ................................................................................................. 41
Chapter 5 – Design Verification .................................................................................................................. 54
5.1 Syringe Selection ............................................................................................................................... 54
5.2 Quantification of Displaced Volume ................................................................................................. 56
5.2.1 Experimental Procedure ............................................................................................................ 57
5.2.2 Experimental Results.................................................................................................................. 57
5.3 Uniformity of Tubing Distension ....................................................................................................... 58
5.3.1 Experimental Procedure ............................................................................................................ 58
5.3.2 Experimental Results.................................................................................................................. 59
5.4 Tissue Ring Loading ........................................................................................................................... 59
5.4.1 Experimental Procedure ............................................................................................................ 59
5.4.2 Experimental Results.................................................................................................................. 60
5.5 Preliminary Impacts of Mechanical Conditioning ............................................................................. 60
5.5.1 Experimental Procedure ............................................................................................................ 60
5.5.2 Experimental Results.................................................................................................................. 61
5.6 Histological Analysis .......................................................................................................................... 64
Chapter 6 - Discussion................................................................................................................................. 67
6.1 Bioreactor design .............................................................................................................................. 67
6.2 Economic impact ............................................................................................................................... 68
6.3 Environmental impact ....................................................................................................................... 69
6.4 Societal influence .............................................................................................................................. 69
2|Page