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Download MembranePro Functional Protein Expression System
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Contents Kit Contents and Storage ................................................................................................................................ 1 Description of the System ............................................................................................. 5 MembranePro™ Functional Protein Expression .......................................................................................... 5 Methods ........................................................................................................................ 9 Generate the pEF6 Expression Construct ..................................................................................................... 9 Transfect 293FT Cells .................................................................................................................................... 12 Harvest Virus-Like Particles (VLPs) ........................................................................................................... 16 Expected Results ............................................................................................................................................ 18 Troubleshooting............................................................................................................................................. 19 Appendix ..................................................................................................................... 22 Scalable Production of Virus-Like Particles (VLPs) in FreeStyle™ 293-F Cells...................................... 22 pEF6/V5-His-TOPO® Vector ....................................................................................................................... 27 Ordering Information ................................................................................................................................... 29 Technical Support .......................................................................................................................................... 31 Purchaser Notification .................................................................................................................................. 32 References ....................................................................................................................................................... 33 i
