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CHAPTER 5 - GERMPLASM CHARACTERIZATION AND EVALUATION ...... 34
Introduction to germplasm characterization and evaluation ........................................ 34
Selection of Materials for Characterization ................................................................. 34
Plant Establishment ................................................................................................... 34
Morpho-Agronomic Characterization.......................................................................... 34
CHAPTER 6 - GERMPLASM EXCHANGE ........................................................ 41
Receipt of germplasm ................................................................................................ 41
Processing newly received germplasm ...................................................................... 41
Protocol for seed distribution...................................................................................... 42
IRRI’s policy on germplasm exchange ....................................................................... 44
IRRI’s Policy on Intellectual Property Rights .............................................................. 44
Protocol for requesting information............................................................................. 44
CHAPTER 7 - DOCUMENTATION AND EXCHANGE OF GERMPLASM
INFORMATION................................................................................................... 46
Documentation and exchange of germplasm information........................................... 46
Background................................................................................................................ 46
System accessibility ................................................................................................... 46
System objectives ...................................................................................................... 46
System menu structure .............................................................................................. 47
Features and capabilities ........................................................................................... 47
IRCGIS User's Manual ............................................................................................... 47
Data integrity.............................................................................................................. 48
Data security.............................................................................................................. 48
Data exchange........................................................................................................... 48
System documentation............................................................................................... 48
CHAPTER 8 - MOLECULAR MARKER LABORATORY................................... 49
Molecular marker laboratory....................................................................................... 49
Protocol for DNA Sample Preparation........................................................................ 49
RAPD Protocol........................................................................................................... 51
Microsatellite Protocol ................................................................................................ 53
AFLP Protocol............................................................................................................ 57
Abbreviations/Acronyms ............................................................................................ 61
CHAPTER 9 - CONSERVATION SUPPORT ..................................................... 65
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