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Table of Contents
Introduction: ………………………………………………………………………………………………………………………………………………………………….. 3
Materials Provided: ……………………………………………………………………………………………………………………………………………….……….. 4
Materials Required for Testing but Not Supplied With OptiMax™ ELISA Kit: …………………………………………………………….…….. 5
Equipment Required: …………………………………………………………………………………………………………………………………..…………………. 5
Unique Considerations for Optimiser™ Microplate: ………………………………………………………………………………………….……………. 6
The Optimiser™ Plate and Assembly: …………………………………………………………………………………………………………….…………….…. 6
Pipetting for Optimiser™-Based Assays: …………………………………………………………………………………………..……………………………. 6
Reader Settings: …………………………………………………………………………………………………………………………………………………….………. 7
Principle of Method: ………………………………………………………………………………………………………………………….…………………….……. 8
Reagent Preparation: ………………………………………………………………………………………………………………………….…………………….…… 8
Procedure: ………………………………………………………………………………………………………………………………………………….………………… 13
Calculations: …………………………………………………………………………………………………………………………………………………………………. 14
Typical Data: ……………………………………………………………………………………………………………………………………………….………………… 15
Standard Curve: ………………………………………………………………………………………………………………………………………………………..….. 15
Precision and Recovery: ………………………………………………………………………………………………………………..……………………………… 15
Limit of Detection: ………………………………………………………………………………………………………………………………………..……………… 16
Detection of Native Protein: ………………………………………………………………………………………………………………………………………… 16
Troubleshooting: ……………………………………………………………………………………………………………………………………..………………….. 17
Alternative OptiMax™ ELISA Procedures: ……………………………………………………………………………………………..……………………… 19
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Intended Use:
Optimiser™ microplates and OptiMax™ ELISA kits (Products) are warranted to perform in conformance with published
product specifications in effect at the time of sale as set forth in product documentation and/or User Manuals. Products
are supplied for Research Use Only. The use of this product for any clinical diagnostic applications is expressly
prohibited. The warranty provided herein is valid only when used by properly trained individuals and does not extend to
anyone other than the original purchaser. No other warranties express or implied, are granted, including without
limitation, implied warranties of merchantability, fitness for any particular purpose, or non-infringement. Buyers’
exclusive remedy for non-conforming product during the warranty period is limited to replacement of or refund for the
non-conforming product.
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