Download FeliX32 Operation Manual

To use EXCORR, the XCorr icon must be present in the hardware configuration. Double
click on this icon, then uncheck the box for Rhodamine Quantum Counter. The
Excitation Correction check box must be enabled in the dialog from the hardware
configuration menu under PHB1 Configure Corrections, and the proper correction
lookup table must be selected. In most cases, this is the lookup table provided by PTI.
Note. The EXCORR curve provided by PTI is fully valid at the 5-nm slit at which it was
measured. If narrower slit widths are used, the EXCORR curve may need to be measured
again at the slit width required for your measurement. Please call PTI for details.
In addition, Real Time Correction must be enabled in the Additional Acquisition
Setup Controls dialog (opened by clicking the More button in an experiment dialog).
The gain should be set correctly as well. This is set by clicking the Reference Source
Gain button to display the XCorr Gain Control dialog. Enter the Excitation
Wavelength into the edit box, and press the Goto button. The excitation wavelength is
generally the excitation wavelength that gives the largest emission value (for scans), or
the wavelength for the timebased experiment. Move the slider control until the Signal
measures ~1 volt. Press Ok to save the changes.
The program will display the corrected curve, the raw data, and the raw photodiode
output upon acquiring data.
During scanning, EXCORR will correct for fluctuations in illumination output due to the
lamp power envelope and variations in the arc itself
Emission Correction (EMCORR)
The emission correction is much simpler than excitation correction. The data trace,
which may already be excitation corrected, is multiplied by the correction file provided
by PTI. This correction is intended to compensate in wavelength dependant variations in
the system on the emission channel. Each emission channel grating has its own
EMCORR file. The EMCORR file is generated by comparison of the emission channel
response to the spectrum of a NIST traceable tungsten light.
Note. If the grating, PMT voltage, or PMT is changed, the Emission Correction should
be re-calibrated for best results. The emission channels will also undergo changes as they
age, and the Emission Correction should also be re-evaluated periodically. Please contact
PTI for details.
To use the EMCORR file in real-time, the Emission Correction check box must be
enabled in the dialog from the hardware configuration menu under PHB1 Configure
Corrections, and the proper correction lookup table must be selected. This lookup table
is provided by PTI, generally with the names emcorri (for channel 1) or emcorrii (for
channel 2). These must be matched with the proper channel, and are properly configured
by PTI personnel during installation. Next, turn on the Real Time Correction in the
Additional Acquisition Setup Controls dialog (opened by clicking the More button in
an experiment dialog) and selecting Emission. The raw and corrected traces will be
displayed.
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