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Chapter 7: Maintenance
Figure 7-2: Orifice box in flask for sonication
7.
Fill the sonicator with enough water so that the level will be higher than the level of
fluid in the flask when the beaker is placed in the sonicator. If your sonicator has a
minimum fill level, be sure to fill up to that level.
8.
Place the flask, with the shearing orifice box into the sonicator. If necessary, use a
weight to keep the container submerged.
Figure 7-3: Flask in sonicator
9.
Visually inspect the shearing devices to determine if any air bubbles are present in
the openings of the orifice box. If air bubbles are present, keep the orifice box
submerged and use a micropipette with a sterile tip to either dislodge or aspirate the
bubble. Take care not to contact the ruby in the center of the orifice box.
10.
Sonicate the submerged orifice box for 10-15 minutes.
11.
After sonication, pour the SDS solution out of the flask.
12.
Leave the orifice box in the flask and rinse with ultrapure distilled water.
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