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Coefficient (R2) and the slope of the curve values are the closest possible to 1
and -3.33, respectively (Figure 3).
Results are considered acceptable, when the efficiency of the amplification is
between 85 – 110% (slope approximately -3.75 - -3.10) and the Correlation
Coefficient value is not less than 0.990.
Figure 2: Post run data analysis: amplification graph displayed in logarithmic scale on the
Applied Biosystems 7300 Real Time PCR System with SDS software version 1.2.3.
Figure 3: Post run data analysis, standard curve on the Applied Biosystems 7300 Real
Time PCR System with SDS software version 1.2.3.
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RQ-S61-48_EN.doc