Download User Manual P/n: RAB108EA

Description & Technology
Measuring principles
3.3. WBC and differential count
3.3.1. General counting principles
The WBC count is carried out twice by two different analysis methods which utilize the total
WBC count in both areas:
◆
Once in the BAS count chamber during the same time as the BAS count,
◆
Once in the optical chamber during the same time as the LMNE count.
The WBC reference count is the count which is obtained from the WBC/BAS count chamber.
3.3.2. BAS/WBC Count
The measurement principle is exactly
the same as the RBC/PLT measurement.
The Differentiation between the BASO’s
and the other leukocytes is obtained by
the use of the ABX BASOLYSE II reagent
with it specific lysing action.
All the WBCs are counted between the
electronic thresholds from <0> to
<BA3>.
The basophils are counted between
thresholds <BA2> and <BA3>.
Fig. 6–17 WBC/BAS histogram
Technical characteristics of the WBC/BAS counts
Initial blood volume
10 µl (CBC or CBC/DIFF)
Method
Impedance
Vol. ABX BASOLYSE II
2000 µl
Aperture diameter
80 µm
Final dilution rate**
1/200
Count vacuum
200 mb
Temperature of reaction
35°C
Count period
2 X 6 seconds
Tab. 6–3: WBC/BAS count
▼
Results
WBC: The number of cells counted within a specified amount of time per volume x WBC coefficient of calibration.
BAS: The number of cells counted within a specified amount of time per volume x the WBC
calibration coefficient in a percentage as to the total number of leukocytes (Basophils and
WBC nuclei)
Pentra 80 - User Manual - RAB108EA
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