Download PowerPlex® Y System

tmd018.0512:EIVD_TM.qxd
5/3/2012
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Appendix ...................................................................................................................................50
A. Advantages of Using the Loci in the PowerPlex® Y System ...................................50
B. DNA Extraction and Quantitation Methods and Automation Support................52
C. The Internal Lane Standard 600...................................................................................52
D. Preparing the PowerPlex® Y System PCR Amplification Mix................................53
E. Composition of Buffers and Solutions........................................................................54
F.
Related Products ............................................................................................................54
Description
STR (short tandem repeat) loci consist of short, repetitive sequence elements 3–7 base
pairs in length (1–4). These repeats are well distributed throughout the human
genome and are a rich source of highly polymorphic markers, which may be detected
using the polymerase chain reaction (5–8). Alleles of STR loci are differentiated by
the number of copies of the repeat sequence contained within the amplified region
and are distinguished from one another using fluorescence detection following
electrophoretic separation.
STR markers on the Y chromosome (Y-STR) have qualities that are distinct from
autosomal markers and are useful for human identification (9–15). Y-STR markers
are found on the nonrecombining region of the Y chromosome (NRY) and produce a
haploid profile when amplified from male DNA. This quality simplifies male/female
mixture interpretation by removing the female contribution from an amplification
profile (16,17). Strict paternal inheritance of these markers makes them useful for
paternity and kinship studies as well.
The PowerPlex® Y System(a,b) is used for human identification applications including
forensic analysis, relationship testing and research use. This system allows coamplification and three-color detection of twelve loci: DYS19, DYS385a/b,
DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439 (18).
One primer specific for each of the DYS389I/II, DYS391 and DYS439 loci is labeled
with fluorescein (FL); one primer specific for each of the DYS385a/b, DYS390 and
DYS393 loci is labeled with carboxy-tetramethyl-rhodamine (TMR); and one primer
specific for each of the DYS19, DYS392, DYS437 and DYS438 loci is labeled with
6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All twelve loci are
amplified simultaneously in a single tube and analyzed in a single injection or gel
lane.
The PowerPlex® Y System is compatible with the ABI PRISM® 310, 3100 and 3100Avant Genetic Analyzers, Applied Biosystems 3130 and 3130xl Genetic Analyzers
and ABI PRISM® 377 DNA Sequencer. The protocols presented in this manual were
tested at Promega Corporation. Amplification and detection instrumentation may
vary. You may need to optimize protocols including cycle number and injection
conditions (or loading volume) for each laboratory instrument. In-house validation
should be performed.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TMD018
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Printed in USA.
Revised 5/12