Download Operating Manual

Using Swinging-Bucket Rotors
Rotor Preparation and Loading
Table 5.1 General Specifications for Beckman Coulter Preparative Swinging-Bucket Rotorsa
k¢ Factorsc
(g/mL)
r = 1.3
(g/mL)
r = 1.5
(g/mL)
r = 1.7
Number of
Tubes ´
Tube
Capacity
(mL)
46
45
48
126
126
135
116
115
123
112
111
118
3´5
6´4
6´5
59.7
67.4
66.7
59
124
137
165
335
368
151
307
338
145
295
325
6´5
6 ´ 13.2
6 ´ 14
109.7
113.6
99.2
99.2
122.1
66.8
64.4
75.3
75.3
72.9
204
228
138
138
276
468
613
393
393
757
428
560
360
360
694
412
536
346
346
668
6 ´ 38.5
6 ´ 17
6´8
6 ´ 20
6 ´ 17
118.2
92.7
75.3
56.2
246
337
680
917
622
840
600
809
6 ´ 38.5
3 ´ 34
Radial Distances (mm)
Rotor
Maximum
Speedb
(rpm)
Relative
Centrifugal
Field (´ g)
at rmax
(SW 65 Ti)
SW 60 Ti
SW 55 Ti
65,000
60,000
55,000
421,000
485,000
368,000
89.0
120.3
108.5
65.1
91.7
84.6
41.2
63.1
60.8
(SW 50.1)
SW 41 Ti
SW 40 Ti
50,000
41,000
40,000
300,000
288,000
285,000
107.3
153.1
158.8
83.5
110.2
112.7
SW 32 Ti
SW 32.1 Ti
(SW 30.1)
(SW 30)
SW 28.1d
32,000
32,000
30,000
30,000
28,000
175,000
187,000
124,000
124,000
150,000
152.5
162.8
123.0
123.0
171.3
SW 28d
(SW 25.1)
28,000
25,000
141,000
90,400
161.0
129.2
rmax
rav
rmin
k
Factor
a. Rotors listed in parentheses are no longer manufactured
b. Maximum speeds are based on a solution density of 1.2 g/mL in all swinging-bucket rotors.
c. Calculated for 5 to 20% (wt/wt) sucrose at 5°C, using the tables in Appendix I of Techniques of Preparative, Zonal, and Continuous Flow
Ultracentrifugation (publication DS-468).
d. SW 28.1M and SW 28M rotors (no longer manufactured) were specially modified versions of the SW 28.1 and SW 28 rotors, and are equipped
with a mechanical overspeed system. These rotors are otherwise identical to the SW 28.1 and SW 28 rotors.
NOTE Although rotor components and accessories made by other manufacturers may fit in the Beckman
Coulter rotor you are using, their safety in the rotor cannot be ascertained by Beckman Coulter. Use of
other manufacturers’ components or accessories in a Beckman Coulter rotor may void the rotor warranty,
and should be prohibited by your laboratory safety officer. Only the components and accessories listed
in the applicable rotor manual should be used.
Tubes and Bottles
Swinging-bucket rotors can accommodate a variety of tube types, listed in the applicable rotor
manual. Refer to CHAPTER 3 for tube filling and sealing or capping requirements. Observe the
maximum rotor speeds and fill volumes listed in the rotor instruction manual.
Rotor Preparation and Loading
For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.
NOTE All buckets, loaded or empty, must be positioned on the rotor body for every run.
LR-IM-24AC
5-3
5