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Using Swinging-Bucket Rotors Rotor Preparation and Loading 3 Remove the bucket gaskets or O-rings and coat them lightly but evenly with silicone vacuum grease (335148). a. Install gaskets or O-rings in the buckets. Cap Gasket Bucket CAUTION Never run a filled bucket without a gasket or O-ring, as the bucket contents may be lost, leading to rotor imbalance and possible failure. 4 Dry the exterior of the tubes. • (Moisture between the tube and the bucket may lead to tube collapse and increase the force required to extract the tube.) a. Slide the filled and sealed tubes into the buckets. • 5 Loaded buckets can be supported in the bucket holder rack available for each rotor. Use the required spacers and/or floating spacers, if necessary, to complete the loading operation. a. If OptiSeal tubes are being used, install a spacer over each plugged tube (refer to the applicable rotor manual). 1) Leave buckets without tubes completely empty. Spacer Tube Plug Tube LR-IM-24AC 5-5 5