Download Operator`s Manual

54 | Chapter 4: Instrument Qualification
Prepare the Plate
1.
Prepare the 75% Test Solution by mixing 15 mL of the 100% Test Solution
with 5 mL of the PBS solution.
2.
Prepare the 50% Test Solution by mixing 10 mL of the 100% Test Solution
with 10 mL of the PBS solution.
3.
Pipette the three solutions into the new 96-well microplate:
¾ 150 µL of the 100% Test Solution into all wells of columns 1 and 2
¾ 150 µL of the 75% Test Solution into all wells of columns 3 and 4
¾ 150 µL of the 50% Test Solution into all wells of columns 5 and 6
Important! After pipetting the diluted test solution
into the microplate and before reading the plate, we
strongly recommend shaking the plate at Variable
speed for four minutes. This will allow any air bubbles
in the solution to settle and the meniscus to stabilize.
Alternatively, wait 20 minutes after pipetting the
diluted test solution before reading the plate.
Read the Plate
1.
Read the microplate five times using Normal mode, single wavelength at
340 nm, no blanking (or blank on air).
2.
Print the five sets of raw data or export it to an Excel spreadsheet using
Gen5.
BioTek Instruments, Inc.