Download WINWCP User Guide V3.9 - Spider

WinWCP V3.9 User Guide
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You can change the number of pulses used to compute the
“LEAK” record and division factor by altering the values in
the boxes shown. The default values are 4 leak records with
voltage waveform divided by –4.
Note that subtraction of the “LEAK” from “TEST” records is done using the leak subtraction
module. (See section 13 for details.)
8.7. Protocol linking
Recording normally stops when the requested sequence of records
within a protocol is completed. Protocols can, however, be linked
together by selecting a protocol from the Link to next protocol
list, so that on completion of the first protocol, control is
transferred to the linked protocol.
8.8. Saving and loading stimulus protocols
When you have created a stimulus protocol, you
can save it to a protocol file by clicking the Save
As button to get the Save Stimulus Protocol
dialog box.
Stimulus protocols are stored as files with .VPR
file extensions, in the directory, C:\
\WinWCP\vprot\
Protocol files can be re-loaded into the Stimulus Generator for editing, by clicking the Open
button, and selecting a protocol file from the list presented in the Load Stimulus Protocol
dialog box.
8.9. Stimulus protocol examples
A number of example protocols are installed in the vprot folder when WinWCP is installed.
Steps.vpr
A family of 12 depolarising, 500 msec duration, voltage steps, ranging
from 10 mV to 120 mV.
TailCur.vpr
A family of 2-step pulse protocol for recording tail currents. A 500 msec
pre-pulse, followed by a 60 mV, 50 msec duration test pulse. The pre-pulse
steps from 10mV to 120 mV. The recording sweep is of 70 msec duration
and begins 10 msec before the test pulse.
Ramp.vpr
A voltage ramp, slewing from –100 mV to +100 mV over a period of 1 sec.
Ap.vpr
A digitised action potential waveform.
Digpulse
A digital stimulus program controlling digital outputs 0 and 1. Dig.0 is
OFF initially and pulses ON for a period of 50 msec, after a delay of 100
msec. Dig. 0 is ON initially and pulses OFF for 50 msec.