Download Troubleshooting Common HPLC Problems

HPLC
Performance Monitoring
Use Your Test Method
(Known Performance)
Troubleshooting
* Monitor at least One Peak in one injection
- Plate Count (Peak width relative to RT),
- Peak Asymmetry,
- Retention Time and/or Retention parameter
- Relative Retention Time for Critical Pair of Analytes.
- Peak Response
* Inject Multiple Runs
- Precision (at least 5 injections)
- Accuracy (Use Control Samples)
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Troubleshooting
Detector
1. Fucose
2. Galactosamine
3. Glucosamine
4. Galactose
5. Glucose
6. Mannose
300
mV
4
6
Control &
Data
Processing
Problem
5
0.00
5.00
Minutes
20.00
Waste
CHEMISTRY
COLUMN/GUARD COLUMN
SOLVENT
SAMPLE
flows 50-5000µL/min)
PUMP
INJECTOR
DETECTOR
INTEGRATION
Fraction
Collector
a b
cd
Pump
Hardware/
Software
Auto
Sampler
HPLC Column
in Oven
Dr. Shulamit Levin, Medtechnica
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